mbp beads (New England Biolabs)
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Structured Review
New England Biolabs
mbp beads
Mbp Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mbp+resin/Amylose+Resin/pmc13690193-203-0-2
Average 99 stars, based on 7637 article reviews
Mbp Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mbp+resin/Amylose+Resin/pmc13690193-203-0-2
Average 99 stars, based on 7637 article reviews
mbp beads - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "The pathogenicity factor of DNA virus interacts with Arabidopsis deubiquitinases UBP12/13 to promote viral infection"
Article Title: The pathogenicity factor of DNA virus interacts with Arabidopsis deubiquitinases UBP12/13 to promote viral infection
Journal: PLOS Pathogens
doi: 10.1371/journal.ppat.1014686
Figure Legend Snippet: (A) Yeast two-hybrid (Y2H) assay of UBP12/13 and CaMV proteins. Yeast co-transformants grown on nonselective media (-Leu/-Trp) and selective media (-Leu/-Trp/-His/-Ade) were shown. Vet.: empty vector. SD/-L.T indicates Leu and Trp drop-out medium. SD/-L.T.H.A indicates Leu, Trp, His and Ade drop-out medium. (B) In vitro pull-down assay shows a direct interaction between GST-UBP12/13 and MBP-P6. GST-UBP12, GST-UBP13 and GST (negative control) were used as baits. MBP-P6 were used as target protein. G: GST. (C) Co-immunoprecipitation (Co-IP) assay for detecting the interaction between UBP12/13 and P6 using transgenic Arabidopsis co-expressing P6 and UBP12/13. Extracts were immunoprecipitated with anti-FLAG antibody and anti-IgG antibody (negative control). Immunoprecipitates were then analyzed by immunoblotting assay using anti-GFP and anti-FLAG antibody. Input controls were shown. (D) Bimolecular fluorescence complementation (BiFC) assays between UBP12/13 and P6 in Arabidopsis protoplasts. Confocal imaging was performed at 12 h post inoculation. UBP12/13 and P6 were fused to nEYFP and cEYFP, respectively. mCherry-VirD2NLS was co-expressed as a nuclear marker. Bar, 5 μm.
Techniques Used: Yeast, Y2H Assay, Plasmid Preparation, In Vitro, Pull Down Assay, Negative Control, Co-Immunoprecipitation Assay, Transgenic Assay, Expressing, Immunoprecipitation, Western Blot, Fluorescence, Confocal, Imaging, Marker
Figure Legend Snippet: (A) In vitro pull-down assay shows a direct interaction between GST-UBP12/13 and MBP-AC2. GST-UBP12, GST-UBP13 and GST (control) were used as baits. MBP-AC2 was used as the target protein. G: GST. (B) Co-immunoprecipitation (Co-IP) assay for detecting the interaction between AC2 and UBP12/13 using the transgenic Arabidopsis co-expressing AC2 and UBP12/13. Extracts were immunoprecipitated with anti-FLAG antibody and anti-IgG antibody. Immunoprecipitates were then analyzed by immunoblotting assay using anti-MYC and anti-FLAG antibody. Input proteins were shown. (C) BiFC assays between UBP12/13 and AC2 in N. benthamiana . Confocal images were taken 2 days after infiltration. UBP12/13 and AC2 were respectively fused to nEYFP and cEYFP. H2B-mCherry was as a nuclear protein marker. Bar, 20 μm.
Techniques Used: In Vitro, Pull Down Assay, Control, Co-Immunoprecipitation Assay, Transgenic Assay, Expressing, Immunoprecipitation, Western Blot, Confocal, Marker
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